Pseudorabies virus (gB) PCR kuzindikira zida

Kufotokozera Kwachidule:

Chikwamachi chimagwiritsa ntchito njira ya PCR yowunikira nthawi yeniyeni kuti izindikire RNA ya Pseudorabies virus (gB gene) (PRV) mu zida zamatenda monga matani, ma lymph node ndi ndulu ndi zida zamatenda zamadzimadzi monga katemera ndi magazi a nkhumba.


Mankhwala Mwatsatanetsatane

Zogulitsa

Dzina la Zogulitsa

Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)

Kukula

Zotsatira za 48 / kit, 50tests / kit

Kugwiritsa Ntchito

Chikwamachi chimagwiritsa ntchito njira ya PCR yowunikira nthawi yeniyeni kuti izindikire RNA ya Pseudorabies virus (gB gene) (PRV) mu zida zamatenda monga matani, ma lymph node ndi ndulu ndi zida zamatenda zamadzimadzi monga katemera ndi magazi a nkhumba. Ndizoyenera kudziwa, kuzindikira komanso kufufuzira za matenda a Pseudorabies virus (gB gene). Chikwamacho ndi ZONSE ZONSE ZOKHUDZA PCR SYSTEM (Lyophilized), yomwe imakhala ndi enzyme ya DNA yokulitsa, poyankha poyambira, zoyambira zapadera ndi ma probes ofunikira pakuwunika kwa fulorosenti ya RT-PCR.

Zamkatimu Zogulitsa

Zigawo Phukusi mfundo Zosakaniza
Kusintha kwa PRV PCR 1 × botolo (Lyophilized ufa)  50Mayeso dNTPs, MgCl2, Oyambirira, Ma probes, Taq DNA polima
6 × 0.2ml 8 bwino Mzere chubu(Wolemba) 48Mayeso
Kulamulira Kwabwino 1 * 0.2ml chubu (lyophilized)  10Mayeso

Plasmid kapena Pseudovirus yokhala ndi zidutswa za PRV (gB)

Kuthetsa yankho 1.5 ml Cryotube 500uL /
Kusagwirizana 1.5 ml Cryotube 200uL 0.9% NaCl

Yosungirako & alumali Moyo

(1) Chikwamacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani chizindikirocho paketi patsiku lopanga ndi tsiku loti lidzathe ntchito.

(4) Mpweya wa lyophilized powder reagent uyenera kusungidwa pa -20 ℃ utatha ndikuwunikanso mobwerezabwereza -thaw ayenera kukhala ochepera kanayi.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya PCR chida.

Chithunzi Chojambula

a) Mtundu wa botolo:

1

b) Mtundu wa chubu wabwino wa 8:

2

Kukula kwa Pcr

Kukhazikitsa Kotsimikizika

Gawo

Zolimbikitsa

Kutentha (℃)

Nthawi

Njira ya kuwala

1

1

95

2min

/

2

40

95

5s

/

60

Ma 10

Sungani kuwala kwa FAM

* Chidziwitso: Zizindikiro za FAM fluorescence njira zisonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira Zoyeserera

Kanema

Kutanthauzira kwa zotsatira

FAM Channel

Ct≤35

PRV (gB) Zabwino

Pansi

PRV (gB) Wachisoni

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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