Porcine Circovirus mtundu 2 PCR kuzindikira zida

Kufotokozera Kwachidule:

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina lazogulitsa

Porcine circovirus Type 2 PCR Detection Kit (Lyophilized)

Kukula

48 mayeso / zida, 50 mayeso / zida

Cholinga Chogwiritsidwa Ntchito

Izi zimagwiritsa ntchito njira yeniyeni ya fulorosenti ya PCR kuti izindikire RNA ya Porcine circovirus mtundu wa 2 (PCV2) mu zipangizo za matenda a minofu monga tonsils, lymph nodes ndi ndulu ndi zipangizo zamadzimadzi monga katemera ndi magazi.Ndikoyenera kufufuza, kufufuza ndi kufufuza matenda a Porcine circovirus mtundu wa 2. Chidacho ndi ALL-READY PCR SYSTEM (Lyophilized), yomwe ili ndi DNA amplification enzyme, reaction buffer, primers ndi probes zofunika kuti azindikire fulorosenti ya PCR. .

Zamkatimu Zamalonda

Zigawo	Phukusi	kufotokoza	Zosakaniza
PCV2 PCR Mix	1 × botolo (Lyophilized ufa)	50 Mayeso	dNTPs, MgCl₂, Zoyamba, Probes, Taq DNA polymerase
PCV2 PCR Mix	6 × 0.2ml 8 chubu bwino(Lyophilized)	48 Mayeso	dNTPs, MgCl₂, Zoyamba, Probes, Taq DNA polymerase
Kulamulira Kwabwino	1 * 0.2 ml chubu (lyophilized)	10 Mayeso	Plasmid kapena Pseudovirus PCV2 yokhala ndi tizidutswa tapadera
Kutha njira	1.5 ml Cryotube	500uL	/
Kuwongolera Koyipa	1.5 ml Cryotube	200uL	0.9% NaCl

Kusungirako & Moyo Walumali

(1) Chidacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.

(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.

Chithunzi cha Opaleshoni

a) Mtundu wa botolo:

b) Mtundu wa chubu wa 8 bwino:

Kukulitsa kwa PC

Zokonda zovomerezeka

Khwerero	Kuzungulira	Kutentha (℃)	Nthawi	Njira ya Fluorescence
1	1	95	2 min	/
2	40	95	5s	/
2	40	60	10s	Sungani ma fluorescence a FAM

*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira za Mayeso

Channel	Kutanthauzira zotsatira
FAM Channel	Kutanthauzira zotsatira
Ct≤35	Vibrio Parahaemolyticus Positive
Undet	Vibrio Parahaemolyticus Negative
35	Kubwereza kokayikitsa, yesaninso*

*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.

Zam'mbuyo: Kachilombo ka matenda a phazi ndi mkamwa RT-PCR Detection Kit

Ena: RT-PCR yodziwira zida za matenda otsekula m'mimba