Mliri wa nkhumba matenda otsekula m'mimba a RT-PCR

Kufotokozera Kwachidule:

Chikwamachi chimagwiritsa ntchito njira ya fulorosenti ya RT-PCR ya nthawi yeniyeni kuti izindikire RNA ya mliri wa matenda otsekula m'mimba a Porcine (PEDV) mu zida zamatenda monga matani, ma lymph node ndi ndulu ndi zida zamatenda zamadzimadzi monga katemera ndi magazi a nkhumba.


Mankhwala Mwatsatanetsatane

Zogulitsa

Dzina la Zogulitsa

Mliri wa nkhuku matenda otsekula m'mimba RT-PCR Detection Kit (Lyophilized)

Kukula

Zotsatira za 48 / kit, 50tests / kit

Kugwiritsa Ntchito

Chikwamachi chimagwiritsa ntchito njira ya fulorosenti ya RT-PCR ya nthawi yeniyeni kuti izindikire RNA ya mliri wa matenda otsekula m'mimba a Porcine (PEDV) mu zida zamatenda monga matani, ma lymph node ndi ndulu ndi zida zamatenda zamadzimadzi monga katemera ndi magazi a nkhumba. Ndi oyenera kuti azindikire, azindikire komanso kufufuzira za matenda a matenda a m'mimba a Porcine. Chikwamacho ndi ZONSE ZONSE ZOKHUDZA PCR SYSTEM (Lyophilized), yomwe imakhala ndi reverse transcriptase, enzyme ya DNA yolimbikitsira, chosinthira choyankha, zoyambira zenizeni ndi ma probes ofunikira kuti RT-PCR ipezeke.

Zamkatimu Zogulitsa

Zigawo Phukusi mfundo Zosakaniza
Kusakaniza kwa PEDV PCR 1 × botolo (Lyophilized ufa)  50Mayeso dNTPs, MgCl2, Oyambirira, Ma probes, Taq DNA polima
6 × 0.2ml 8 bwino Mzere chubu(Wolemba) 48Mayeso
Kulamulira Kwabwino 1 * 0.2ml chubu (lyophilized)  10Mayeso

Plasmid kapena Pseudovirus yokhala ndi zidutswa za PEDV

Kuthetsa yankho 1.5 ml Cryotube 500uL /
Kusagwirizana 1.5 ml Cryotube 200uL 0.9% NaCl

Yosungirako & alumali Moyo

(1) Chikwamacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani chizindikirocho paketi patsiku lopanga ndi tsiku loti lidzathe ntchito.

(4) Mpweya wa lyophilized powder reagent uyenera kusungidwa pa -20 ℃ utatha ndikuwunikanso mobwerezabwereza -thaw ayenera kukhala ochepera kanayi.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya PCR chida.

Chithunzi Chojambula

a) Mtundu wa botolo:

1

b) Mtundu wa chubu wabwino wa 8:

2

Kukula kwa Pcr

Kukhazikitsa Kotsimikizika

Gawo

Zolimbikitsa

Kutentha (℃)

Nthawi

Njira ya kuwala

1

1

48

Mphindi 8

/

2

1

95

2min

/

3

40

95

5s

/

60

Ma 10

Sungani kuwala kwa FAM

* Chidziwitso: Zizindikiro za FAM fluorescence njira zisonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira Zoyeserera

Kanema

Kutanthauzira kwa zotsatira

FAM Channel

Ct≤35

PEDV Yabwino

Pansi

PEDV Wachinyengo

35<Ct≤40

Suspicious result, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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