中文RT-PCR yodziwira zida za matenda otsekula m'mimba
Dzina lazogulitsa
Kachilombo koyambitsa matenda otsegula m'mimba RT-PCR (Lyophilized)
Kukula
48 mayeso / zida, 50 mayeso / zida
Cholinga Chogwiritsidwa Ntchito
Chidachi chimagwiritsa ntchito njira yeniyeni ya fulorosenti ya RT-PCR kuti izindikire RNA ya Porcine mliri wa kutsekula m'mimba (PEDV) mu zida za matenda a minofu monga ma tonsils, ma lymph nodes ndi ndulu ndi zida zamadzimadzi monga katemera ndi magazi a nkhumba.Ndiwoyenera kuzindikira, kuzindikira komanso kufufuza matenda a Porcine mliri wa kutsekula m'mimba.Kit ndi ALL-READY PCR SYSTEM(Lyophilized), yomwe ili ndi reverse transcriptase, DNA amplification enzyme, reaction buffer, primers ndi ma probes ofunikira kuti azindikire fulorosenti ya RT-PCR.
Zamkatimu Zamalonda
| Zigawo | Phukusi | kufotokoza | Zosakaniza |
| PEDV PCR Mix | 1 × botolo (Lyophilized ufa) | 50 Mayeso | dNTPs, MgCl2, Zoyamba, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 chubu bwino(Lyophilized) | 48 Mayeso | ||
| Kulamulira Kwabwino | 1 * 0.2 ml chubu (lyophilized) | 10 Mayeso | Plasmid kapena Pseudovirus yokhala ndi zidutswa za PEDV |
| Kutha njira | 1.5 ml Cryotube | 500uL | / |
| Kuwongolera Koyipa | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kusungirako & Moyo Walumali
(1) Chidacho chimatha kunyamulidwa kutentha.
(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.
(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.
(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera zosakwana 4 zina.
Zida
GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.
Chithunzi cha Opaleshoni
a) Mtundu wa botolo:
b) Mtundu wa chubu wa 8 bwino:
Kukulitsa kwa PC
Zokonda zovomerezeka
| Khwerero | Kuzungulira | Kutentha (℃) | Nthawi | Njira ya Fluorescence |
| 1 | 1 | 48 | 8 min | / |
| 2 | 1 | 95 | 2 min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Sungani ma fluorescence a FAM |
*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.
Kutanthauzira Zotsatira za Mayeso
| Channel | Kutanthauzira zotsatira |
| FAM Channel | |
| Ct≤35 | PEDV Positive |
| Undet | PEDV Negative |
| 35 | Zotsatira zokayikitsa, yesaninso* |
*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.
