E.coli O157: Chida chowunikira cha H7 PCR

Kufotokozera Kwachidule:

Escherichia coli O157: H7 (E. coli O157: H7) ndi bakiteriya wopanda gramu wa mtundu wa Enterobacteriaceae, womwe umatulutsa poizoni wambiri wa Vero.


Mankhwala Mwatsatanetsatane

Zogulitsa

Dzina la Zogulitsa

E.coli O157: Chida chodziwika cha H7 PCR (Lyophilized)

Kukula

Zotsatira za 48 / kit, 50tests / kit

Kugwiritsa Ntchito

Escherichia coli O157: H7 (E. coli O157: H7) ndi bakiteriya wopanda gramu wa mtundu wa Enterobacteriaceae, womwe umatulutsa poizoni wambiri wa Vero. Mwachipatala, nthawi zambiri zimachitika mwadzidzidzi ndikumva kupweteka m'mimba komanso kutsekula m'madzi, kutsatiridwa ndi kutsekula m'mimba masiku angapo pambuyo pake, zomwe zimatha kuyambitsa malungo kapena malungo, komanso kufa pamavuto akulu. Izi ndizoyenera kudziwa Escherichia coli O157: H7 mu chakudya, zitsanzo zamadzi, ndowe, masanzi, madzi opititsa patsogolo mabakiteriya ndi zitsanzo zina pogwiritsa ntchito PCR yeniyeni. Lyophilized), yomwe imakhala ndi enzyme ya DNA yokukulitsa, zomwe zimayankha, zoyambira zenizeni ndi ma probes omwe amafunikira kuti PCR ipezeke.

Zamkatimu Zogulitsa

Zigawo Phukusi mfundo Zosakaniza
E.coli O157: H7 PCR Kusakaniza 1 × botolo (Lyophilized ufa)  50Mayeso dNTPs, MgCl2, Oyambirira, Ma probes, Reverse Transcriptase, Taq DNA polima
6 × 0.2ml 8 bwino Mzere chubu(Wolemba) 48Mayeso
Kulamulira Kwabwino 1 * 0.2ml chubu (lyophilized)  10Mayeso

Plasmid yokhala ndi E. coli O157: Zidutswa za H7

Kuthetsa yankho 1.5 ml Cryotube 500uL /
Kusagwirizana 1.5 ml Cryotube 200uL 0.9% NaCl

Yosungirako & alumali Moyo

(1) Chikwamacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani chizindikirocho paketi patsiku lopanga ndi tsiku loti lidzathe ntchito.

(4) Mpweya wa lyophilized powder reagent uyenera kusungidwa pa -20 ℃ utatha ndikuwunikanso mobwerezabwereza -thaw ayenera kukhala ochepera kanayi.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya PCR chida.

Chithunzi Chojambula

a) Mtundu wa botolo:

1

b) Mtundu wa chubu wabwino wa 8:

2

Kukula kwa Pcr

Zalangizidwa Kukhazikitsa

Gawo     Zolimbikitsa Kutentha (℃)   Nthawi Njira ya kuwala
1 1 95 2min  
2 40 95 5s  
60 Ma 10 Sungani kuwala kwa FAM

* Chidziwitso: Chizindikiro cha FAM fluorescence channel chidzasonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira Zoyeserera

Kanema

Kutanthauzira kwa zotsatira

FAM Channel

Ct≤35

E.coli O157: H7 Zabwino

Pansi

E.coli O157: H7 Wachisoni

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


  • Previous:
  • Next:

  • Related Products