中文E.coli O157: H7 PCR yozindikira zida
Dzina lazogulitsa
E.coli O157: H7 PCR kuzindikira zida (Lyophilized)
Kukula
48 mayeso / zida, 50 mayeso / zida
Cholinga Chogwiritsidwa Ntchito
Escherichia coli O157:H7 (E.coli O157:H7) ndi kachilombo ka gram-negative wa mtundu wa Enterobacteriaceae, yemwe amatulutsa kuchuluka kwa poizoni wa Vero.Kachipatala, nthawi zambiri zimachitika mwadzidzidzi ndi ululu waukulu m'mimba ndi kutsekula m'mimba madzi, kutsatiridwa ndi kutsekula m'mimba kwa masiku angapo pambuyo pake, zomwe zingayambitse kutentha thupi kapena kutentha thupi, ndi imfa yoopsa kwambiri.Chidachi ndi choyenera kudziwa za Escherichia coli O157: H7 mu chakudya, zitsanzo za madzi, ndowe, masanzi, madzi owonjezera mabakiteriya ndi zitsanzo zina pogwiritsa ntchito mfundo ya PCR yeniyeni. Lyophilized), yomwe ili ndi DNA amplification enzyme, reaction buffer, primers ndi ma probes ofunikira kuti azindikire PCR ya fulorosenti.
Zamkatimu Zamalonda
| Zigawo | Phukusi | kufotokoza | Zosakaniza |
| E.coli O157:H7 PCR Mix | 1 × botolo (Lyophilized ufa) | 50 Mayeso | dNTPs, MgCl2, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 chubu bwino(Lyophilized) | 48 Mayeso | ||
| Kulamulira Kwabwino | 1 * 0.2 ml chubu (lyophilized) | 10 Mayeso | Plasmid yomwe ili ndi E.coli O157: H7 zidutswa zenizeni |
| Kutha njira | 1.5 ml Cryotube | 500uL | / |
| Kuwongolera Koyipa | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kusungirako & Moyo Walumali
(1) Chidacho chimatha kunyamulidwa kutentha.
(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.
(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.
(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.
Zida
GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.
Chithunzi cha Opaleshoni
a) Mtundu wa botolo:
b) Mtundu wa chubu wa 8 bwino:
Kukulitsa kwa PC
AnalimbikitsaKukhazikitsa
| Khwerero | Kuzungulira | Kutentha (℃) | Nthawi | Njira ya Fluorescence |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Sungani ma fluorescence a FAM |
*Zindikirani: Chizindikiro cha njira ya FAM fluorescence idzasonkhanitsidwa pa 60 ℃.
Kutanthauzira Zotsatira za Mayeso
| Channel | Kutanthauzira zotsatira |
| FAM Channel | |
| Ct≤35 | E.coli O157:H7 Zabwino |
| Undet | E.coli O157:H7 Zoipa |
| 35 | Kubwereza kokayikitsa, yesaninso* |
*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.
