Kachilombo ka matenda a phazi ndi mkamwa RT-PCR Detection Kit

Kachilombo ka matenda a phazi ndi pakamwa RT-PCR Detection Kit Chithunzi Chowonetsedwa

Kufotokozera Kwachidule:

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina lazogulitsa

Kachilombo ka matenda a phazi ndi pakamwa RT-PCR Detection Kit (Lyophilized)

Kukula

48 mayeso / zida, 50 mayeso / zida

Cholinga Chogwiritsidwa Ntchito

Chidachi chimagwiritsa ntchito njira yeniyeni ya fulorosenti ya RT-PCR kuti izindikire RNA ya matenda a phazi ndi pakamwa (CSFV) mu zida za matenda a minofu monga ma tonsils, ma lymph nodes ndi ndulu ndi zida zamadzimadzi monga katemera ndi magazi a nkhumba.Ndiwoyenera kuzindikira, kuzindikira ndi kufufuza matenda a matenda a phazi ndi pakamwa.Chidachi ndi ALL-READY PCR SYSTEM(Lyophilized), yomwe ili ndi DNA amplification enzyme, reverse transcriptase, reaction buffer, primers ndi ma probes ofunikira kuti azindikire PCR ya fulorosenti.

Zamkatimu Zamalonda

Zigawo	Phukusi	kufotokoza	Zosakaniza
Chithunzi cha FMD PCR	1 × botolo (Lyophilized ufa)	50 Mayeso	dNTPs, MgCl₂, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase
Chithunzi cha FMD PCR	6 × 0.2ml 8 chubu bwino(Lyophilized)	48 Mayeso
Kulamulira Kwabwino	1 * 0.2 ml chubu (lyophilized)	10 Mayeso	Plasmid yokhala ndi zidutswa za FMD
Kutha njira	1.5 ml Cryotube	500uL	/
Kuwongolera Koyipa	1.5 ml Cryotube	200uL	0.9% NaCl

Kusungirako & Moyo Walumali

(1) Chidacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.

(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.

Chithunzi cha Opaleshoni

a) Mtundu wa botolo:

b) Mtundu wa chubu wa 8 bwino:

Kukulitsa kwa PC

AnalimbikitsaKukhazikitsa

Khwerero	Kuzungulira	Kutentha (℃)	Nthawi	Njira ya Fluorescence
1	1	50	8 min
2	1	95	2 min
3	40	95	5s
3	40	60	10s	Sungani ma fluorescence a FAM

*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira za Mayeso

Channel	Kutanthauzira zotsatira
FAM Channel	Kutanthauzira zotsatira
Ct≤35	FMD Zabwino
Undet	FMD Negative
35	Zotsatira zokayikitsa, yesaninso*

*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.

Zam'mbuyo: Swine fever virus RT-PCR Detection Kit

Ena: Porcine Circovirus mtundu 2 PCR kuzindikira zida