Porcine Reproductive and Reproductive Syndrome Virus RT-PCR Detection Kit

Kufotokozera Kwachidule:

Chidachi chimagwiritsa ntchito njira yeniyeni ya fulorosenti ya RT-PCR kuti izindikire RNA ya Porcine reproductive and breathing virus virus nucleic acid kit (PRRSV) muzinthu za matenda a minofu monga tonsils, lymph nodes ndi ndulu ndi zipangizo zamadzimadzi monga katemera ndi magazi. wa nkhumba .


Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Dzina lazogulitsa

Porcine reproductive and breathing virus virus RT-PCR kit (Lyophilized)

Kukula

48 mayeso / zida, 50 mayeso / zida

Cholinga Chogwiritsidwa Ntchito

Chidachi chimagwiritsa ntchito njira yeniyeni ya fulorosenti ya RT-PCR kuti izindikire RNA ya Porcine reproductive and breathing virus virus nucleic acid kit (PRRSV) muzinthu za matenda a minofu monga tonsils, lymph nodes ndi ndulu ndi zipangizo zamadzimadzi monga katemera ndi magazi. wa nkhumba .Ndiwoyenera kuzindikira, kuzindikira komanso kufufuza matenda a Porcine Blue Ear Virus.Chidachi ndi ALL-READY PCR SYSTEM(Lyophilized), yomwe ili ndi reverse transcriptase, DNA amplification enzyme, reaction buffer, primers ndi ma probes ofunikira kuti azindikire fulorosenti ya RT-PCR.

Zamkatimu Zamalonda

Zigawo Phukusi kufotokoza Zosakaniza
PBEV PCR Mix 1 × botolo (Lyophilized ufa)  50 Mayeso dNTPs, MgCl2, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 chubu bwino(Lyophilized) 48 Mayeso
Kulamulira Kwabwino 1 * 0.2 ml chubu (lyophilized)  10 Mayeso

Plasmid kapena Pseudovirus yokhala ndi zidutswa za PRRSV

Kutha njira 1.5 ml Cryotube 500uL /
Kuwongolera Koyipa 1.5 ml Cryotube 200uL 0.9% NaCl

Kusungirako & Moyo Walumali

(1) Chidacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.

(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera zosakwana 4 zina.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.

Chithunzi cha Opaleshoni

a) Mtundu wa botolo:

1

b) Mtundu wa chubu wa 8 bwino:

2

Kukulitsa kwa PC

Zokonda zovomerezeka

Khwerero

Kuzungulira

Kutentha (℃)

Nthawi

Njira ya Fluorescence

1

1

48

8 min

/

2

1

95

2 min

/

3

40

95

5s

/

60

10s

Sungani ma fluorescence a FAM

*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira za Mayeso

Channel

Kutanthauzira zotsatira

FAM Channel

Ct≤35

Vibrio Parahaemolyticus Positive

Undet

Vibrio Parahaemolyticus Negative

35

Kubwereza kokayikitsa, yesaninso*

*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.


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