中文Africa swine fever virus PCR kuzindikira zida
Dzina lazogulitsa
Africa swine fever virus PCR kit (Lyophilized)
Kukula
48 mayeso / zida, 50 mayeso / zida
Cholinga Chogwiritsidwa Ntchito
Chidachi chimagwiritsa ntchito njira yeniyeni ya fulorosenti ya PCR kuti izindikire DNA yaAfrica Swine fever virus (ASFV)mu zipangizo matenda minofu monga tonsils, mwanabele ndi ndulu ndi madzi zinthu matenda monga katemera ndi magazi a nkhumba.Ndikoyenera kuzindikira, kuzindikira ndi kufufuza kwa epidemiologicalAfrica swine fever virus.Kit ndi ALL-READY PCR SYSTEM(Lyophilized), yomwe ili ndi DNA amplification enzyme, reaction buffer, primers ndi ma probes ofunikira kuti azindikire fulorosenti ya PCR.
Zamkatimu Zamalonda
| Zigawo | Phukusi | kufotokoza | Zosakaniza |
| ASFV PCR Mix | 1 × botolo (Lyophilized ufa) | 50 Mayeso | dNTPs, MgCl2, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 chubu bwino(Lyophilized) | 48 Mayeso | ||
| Kulamulira Kwabwino | 1 * 0.2 ml chubu (lyophilized) | 10 Mayeso | Plasmid yokhala ndi zidutswa za ASFV |
| Kutha njira | 1.5 ml Cryotube | 500uL | / |
| Kuwongolera Koyipa | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kusungirako & Moyo Walumali
(1) Chidacho chimatha kunyamulidwa kutentha.
(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.
(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.
(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.
Zida
GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.
Chithunzi cha Opaleshoni
a) Mtundu wa botolo:
b) Mtundu wa chubu wa 8 bwino:
Kukulitsa kwa PC
AnalimbikitsaKukhazikitsa
| Khwerero | Kuzungulira | Kutentha (℃) | Nthawi | Njira ya Fluorescence |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Sungani ma fluorescence a FAM |
*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.
Kutanthauzira Zotsatira za Mayeso
| Channel | Kutanthauzira zotsatira |
| FAM Channel | |
| Ct≤35 | ASFV Zabwino |
| Undet | ASFV Negative |
| 35 | Zotsatira zokayikitsa, yesaninso* |
*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.
