Vibrio parahaemolyticus PCR Detection Kit

Kufotokozera Kwachidule:

Vibrio Parahemolyticus (yemwe amadziwikanso kuti Halophile Vibrio Parahemolyticus) ndi Gram-negative polymorphic bacillus kapena Vibrio Parahemolyticus.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina lazogulitsa

Vibrio Parahaemolyticus PCR Detection Kit (Lyophilized)

Kukula

48 mayeso / zida, 50 mayeso / zida

Cholinga Chogwiritsidwa Ntchito

Vibrio Parahemolyticus (yemwe amadziwikanso kuti Halophile Vibrio Parahemolyticus) ndi Gram-negative polymorphic bacillus kapena Vibrio Parahemolyticus. PCR ndipo ndiyoyenera kuzindikira bwino kwa Vibrio Parahaemolyticus muzakudya, zitsanzo zamadzi, ndowe, masanzi, ndi madzi owonjezera. ndi ma probe ofunikira kuti azindikire fulorosenti RT-PCR.

Zamkatimu Zamalonda

Zigawo Phukusi kufotokoza Zosakaniza
PCR Mix 1 × botolo (Lyophilized ufa)  50 Mayeso dNTPs, MgCl2, Zoyamba, Probes, Taq DNA polymerase
6 × 0.2ml 8 chubu bwino(Lyophilized) 48 Mayeso
Kulamulira Kwabwino 1 * 0.2 ml chubu (lyophilized)  10 Mayeso

Plasmid kapena Pseudovirus yokhala ndi tizidutswa tapadera

Kutha njira 1.5 ml Cryotube 500uL /
Kuwongolera Koyipa 1.5 ml Cryotube 100 ul 0.9% NaCl

Kusungirako & Moyo Walumali

(1) Chidacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.

(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.

Chithunzi cha Opaleshoni

a) Mtundu wa botolo:

1

b) Mtundu wa chubu wa 8 bwino:

2

Kukulitsa kwa PC

Zokonda zovomerezeka

Khwerero

Kuzungulira

Kutentha (℃)

Nthawi

Njira ya Fluorescence

1

1

95

2 min

/

2

40

95

6s

/

60

12s

Sungani ma fluorescence a FAM

Kutanthauzira Zotsatira za Mayeso

Channel

Kutanthauzira zotsatira

FAM Channel

Ct≤35

Vibrio Parahaemolyticus Positive

Undet

Vibrio Parahaemolyticus Negative

35

Kubwereza kokayikitsa, yesaninso*

*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.


  • Zam'mbuyo:
  • Ena:

  • Zogwirizana nazo