中文Listeria monocytogenes PCR Detection Kit
Dzina lazogulitsa
Listeria monocytogenes PCR Detection Kit
Kukula
48 mayeso / zida, 50 mayeso / zida
Cholinga Chogwiritsidwa Ntchito
Listeria monocytogenes ndi gram-positive microbacterium yomwe imatha kukula pakati pa 4℃ ndi 45℃.Ndi imodzi mwa tizilombo toyambitsa matenda zomwe zimawopseza thanzi la anthu muzakudya zafriji.Zizindikiro zazikulu za matenda ndi septicemia, meningitis ndi mononucleosis.Chidachi ndi choyenera kudziwa za Listeria monocytogene mu chakudya, zitsanzo za madzi, ndowe, masanzi, madzi owonjezera mabakiteriya ndi zitsanzo zina pogwiritsa ntchito mfundo ya nthawi yeniyeni ya fluorescence PCR. , yomwe ili ndi DNA amplification enzyme, reverse transcriptase, reaction buffer, ma primers enieni ndi ma probes ofunikira kuti azindikire PCR ya fulorosenti.
Kusungirako & Moyo Walumali
(1) Chidacho chimatha kunyamulidwa kutentha.
(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.
(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.
(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.
Zamkatimu Zamalonda
| Zigawo | Phukusi | kufotokoza | Zosakaniza |
| Listeria monocytogenes PCR Mix | 1 × botolo (Lyophilized ufa) | 50 Mayeso | dNTPs, MgCl2, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 chubu bwino(Lyophilized) | 48 Mayeso | ||
| Kulamulira Kwabwino | 1 * 0.2 ml chubu (lyophilized) | 10 Mayeso | Plasmid yokhala ndi zidutswa za Listeria monocytogenes |
| Kutha njira | 1.5 ml Cryotube | 500uL | / |
| Kuwongolera Koyipa | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Zida
GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.
Chithunzi cha Opaleshoni
a)
b)
Kukulitsa kwa PC
AnalimbikitsaKukhazikitsa
| Khwerero | Kuzungulira | Kutentha (℃) | Nthawi | Njira ya Fluorescence |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Sungani ma fluorescence a FAM |
*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.
Kutanthauzira Zotsatira za Mayeso
| Channel | Kutanthauzira zotsatira |
| FAM Channel | |
| Ct≤35 | Listeria monocytogenes zabwino |
| Undet | Listeria monocytogenes Negative |
| 35 | Kubwereza kokayikitsa, yesaninso* |
*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.
