Shigella PCR Detection Kit

Kufotokozera Kwachidule:

Shigella ndi mtundu wa gram-negative brevis bacilli, wa tizilombo toyambitsa matenda a m'mimba, komanso tizilombo toyambitsa matenda a bacillary kamwazi.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina lazogulitsa

Shigella PCR Detection Kit (lyophilized)

Kukula

48 mayeso / zida, 50 mayeso / zida

Cholinga Chogwiritsidwa Ntchito

Shigella ndi mtundu wa gram-negative brevis bacilli, wa tizilombo toyambitsa matenda a m'mimba, komanso tizilombo toyambitsa matenda a bacillary kamwazi.Chidachi chimagwiritsa ntchito mfundo ya PCR yeniyeni ya fulorosenti, yomwe ili yoyenera kutsimikizira Shigella muzakudya, zitsanzo zamadzi, ndowe, masanzi, ndi madzi owonjezera.

Zamkatimu Zamalonda

Zigawo	Phukusi	kufotokoza	Zosakaniza
PCR Mix	1 × botolo (Lyophilized ufa)	50 Mayeso	dNTPs, MgCl₂, Zoyamba, Probes, Taq DNA polymerase
PCR Mix	6 × 0.2ml 8 chubu bwino(Lyophilized)	48 Mayeso	dNTPs, MgCl₂, Zoyamba, Probes, Taq DNA polymerase
Kulamulira Kwabwino	1 * 0.2 ml chubu (lyophilized)	10 Mayesero	Plasmid kapena Pseudovirus yokhala ndi tizidutswa tapadera
Kutha njira	1.5 ml Cryotube	500uL	/
Kuwongolera Koyipa	1.5 ml Cryotube	100 ul	0.9% NaCl

Kusungirako & Moyo Walumali

(1) Chidacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.

(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.

Chithunzi cha Opaleshoni

a) Mtundu wa botolo:

b) Mtundu wa chubu wa 8 bwino:

Kukulitsa kwa PC

Zokonda zovomerezeka

Khwerero	Kuzungulira	Kutentha (℃)	Nthawi	Njira ya Fluorescence
1	1	95	2 min	/
2	40	95	5s	/
2	40	60	10s	Sungani ma fluorescence a FAM

Kutanthauzira Zotsatira za Mayeso

Channel	Kutanthauzira zotsatira
FAM Channel	Kutanthauzira zotsatira
Ct≤35	Shigella Positive
Undet	Shigella Negative
35	Kubwereza kokayikitsa, yesaninso*

*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.

Zam'mbuyo: Salmonella PCR Detection Kit

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