Norovirus (GⅡ) RT-PCR Detection Kit

Kufotokozera Kwachidule:

Ndikoyenera kuzindikira Norovirus (GⅡ) mu nkhono, masamba obiriwira ndi zipatso, madzi, ndowe, masanzi ndi zitsanzo zina.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina lazogulitsa

Norovirus (GⅡ) RT-PCR Detection Kit (Lyophilized)

Kukula

48 mayeso / zida, 50 mayeso / zida

Cholinga Chogwiritsidwa Ntchito

Ndikoyenera kuzindikira Norovirus (GⅡ) mu nkhono, masamba obiriwira ndi zipatso, madzi, ndowe, masanzi ndi zitsanzo zina.Nucleic acid m'zigawo ziyenera kuchitidwa ndi nucleic acid m'zigawo zida kapena mwachindunji pyrolysis njira kutengera mitundu yosiyanasiyana ya zitsanzo. ndi ma probes ofunikira kuti azindikire PCR ya fulorosenti.

Zamkatimu Zamalonda

Zigawo Phukusi kufotokoza Zosakaniza
Norovirus (GⅡ) PCR Mix 1 × botolo (Lyophilized ufa)  50 Mayeso dNTPs, MgCl2, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 chubu bwino(Lyophilized) 48 Mayeso
Kulamulira Kwabwino 1 * 0.2 ml chubu (lyophilized)  10 Mayeso

Plasmid yokhala ndi tiziduswa ta Norovirus (GⅡ).

Kutha njira 1.5 ml Cryotube 500uL /
Kuwongolera Koyipa 1.5 ml Cryotube 200uL 0.9% NaCl

Kusungirako & Moyo Walumali

(1) Chidacho chimatha kunyamulidwa kutentha.

(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.

(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.

(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.

Zida

GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.

Chithunzi cha Opaleshoni

a) Mtundu wa botolo:

1

b) Mtundu wa chubu wa 8 bwino:

2

Kukulitsa kwa PC

AnalimbikitsaKukhazikitsa

Khwerero Kuzungulira Kutentha (℃) Nthawi Njira ya Fluorescence
1 1 50 8 min  
2 1 95 2 min  
3 40 95 5s  
60 10s Sungani ma fluorescence a FAM

*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.

Kutanthauzira Zotsatira za Mayeso

Channel

Kutanthauzira zotsatira

FAM Channel

Ct≤35

Norovirus (GⅡ) Zabwino

Undet

Norovirus (GⅡ) Negative

35

Kubwereza kokayikitsa, yesaninso*

*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.


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