中文Staphylococcus aureus PCR Detection Kit
Dzina lazogulitsa
Staphylococcus aureus PCR Detection Kit (lyophilized)
Kukula
48 mayeso / zida, 50 mayeso / zida
Cholinga Chogwiritsidwa Ntchito
Staphylococcus aureus ndi wa mtundu wa Staphylococcus ndipo ndi bakiteriya wokhala ndi gramu.Ndi tizilombo tating'onoting'ono tomwe timakhala ndi zakudya zomwe zimatha kutulutsa ma enterotoxins ndikuyambitsa poizoni wa chakudya.zida izi amagwiritsa ntchito mfundo zenizeni nthawi fulorosenti PCR ndi oyenera kudziwika Mkhalidwe wa Staphylococcus aureus mu chakudya, zitsanzo madzi, ndowe, masanzi, kulemeretsa madzi ndi zitsanzo zina.
Zamkatimu Zamalonda
| Zigawo | Phukusi | kufotokoza | Zosakaniza |
| PCR Mix | 1 × botolo (Lyophilized ufa) | 50 Mayeso | dNTPs, MgCl2, Zoyamba, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 chubu bwino(Lyophilized) | 48 Mayeso | ||
| Kulamulira Kwabwino | 1 * 0.2 ml chubu (lyophilized) | 10 Mayeso | Plasmid kapena Pseudovirus yokhala ndi tizidutswa tapadera |
| Kutha njira | 1.5 ml Cryotube | 500uL | / |
| Kuwongolera Koyipa | 1.5 ml Cryotube | 100 ul | 0.9% NaCl |
Kusungirako & Moyo Walumali
(1) Chidacho chimatha kunyamulidwa kutentha.
(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.
(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.
(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.
Zida
GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.
Chithunzi cha Opaleshoni
a) Mtundu wa botolo:
b) Mtundu wa chubu wa 8 bwino:
Kukulitsa kwa PC
Zokonda zovomerezeka
| Khwerero | Kuzungulira | Kutentha (℃) | Nthawi | Njira ya Fluorescence |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Sungani ma fluorescence a FAM |
Kutanthauzira Zotsatira za Mayeso
| Channel | Kutanthauzira zotsatira |
| FAMChannel | |
| Ct≤35 | Staphylococcus aureus Zabwino |
| Undet | Staphylococcus aureus Zoipa |
| 35<Ct≤40 | Zokayikitsayambiranso,yesaninso* |
*Ngatindi zotsatira za njira ya FAMhasmtengo wa Ct ≤40ndi kuwonetsa zofananira“S”mawonekedwe amplification curve, zotsatira zake zimatanthauziridwa kukhala zabwino, apo ayi zimakhala zotsutsa.
