中文Norovirus (GⅡ) RT-PCR Detection Kit
Dzina lazogulitsa
Norovirus (GⅡ) RT-PCR Detection Kit (Lyophilized)
Kukula
48 mayeso / zida, 50 mayeso / zida
Cholinga Chogwiritsidwa Ntchito
Ndikoyenera kuzindikira Norovirus (GⅡ) mu nkhono, masamba obiriwira ndi zipatso, madzi, ndowe, masanzi ndi zitsanzo zina.Nucleic acid m'zigawo ziyenera kuchitidwa ndi nucleic acid m'zigawo zida kapena mwachindunji pyrolysis njira kutengera mitundu yosiyanasiyana ya zitsanzo. ndi ma probes ofunikira kuti azindikire PCR ya fulorosenti.
Zamkatimu Zamalonda
| Zigawo | Phukusi | kufotokoza | Zosakaniza |
| Norovirus (GⅡ) PCR Mix | 1 × botolo (Lyophilized ufa) | 50 Mayeso | dNTPs, MgCl2, Zoyamba, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 chubu bwino(Lyophilized) | 48 Mayeso | ||
| Kulamulira Kwabwino | 1 * 0.2 ml chubu (lyophilized) | 10 Mayeso | Plasmid yokhala ndi tiziduswa ta Norovirus (GⅡ). |
| Kutha njira | 1.5 ml Cryotube | 500uL | / |
| Kuwongolera Koyipa | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kusungirako & Moyo Walumali
(1) Chidacho chimatha kunyamulidwa kutentha.
(2) Moyo wa alumali ndi miyezi 18 pa -20 ℃ ndi miyezi 12 pa 2 ℃ ~ 30 ℃.
(3) Onani zolembedwa pa zida za tsiku lopanga ndi tsiku lotha ntchito.
(4) The lyophilized ufa Baibulo reagent ziyenera kusungidwa pa -20 ℃ pambuyo kuvunda ndi mobwerezabwereza amaundana - thaw ayenera kukhala zosakwana 4 zina.
Zida
GENECHECKER UF-150, UF-300 zenizeni nthawi ya fluorescence PCR chida.
Chithunzi cha Opaleshoni
a) Mtundu wa botolo:
b) Mtundu wa chubu wa 8 bwino:
Kukulitsa kwa PC
AnalimbikitsaKukhazikitsa
| Khwerero | Kuzungulira | Kutentha (℃) | Nthawi | Njira ya Fluorescence |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Sungani ma fluorescence a FAM |
*Zindikirani: Zizindikiro zamakanema a FAM fluorescence zidzasonkhanitsidwa pa 60 ℃.
Kutanthauzira Zotsatira za Mayeso
| Channel | Kutanthauzira zotsatira |
| FAM Channel | |
| Ct≤35 | Norovirus (GⅡ) Zabwino |
| Undet | Norovirus (GⅡ) Negative |
| 35 | Kubwereza kokayikitsa, yesaninso* |
*Ngati zotsatira zoyesereranso za tchanelo cha FAM zili ndi mtengo wa Ct ≤40 ndipo zikuwonetsa mapindikidwe okulitsa mawonekedwe a "S", zotsatira zake zimatanthauziridwa kuti zabwino, apo ayi nzopanda pake.
